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Cellular Copper Measurement and Western Blot Analysis of Copper Acetate

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Copper Acetate Manufacturers(WSDTY) proposed premalignant Copper Acetate cells were cultured for 2 weeks in culture media with or without 25 μM CuCl2. Cells were collected and counted to determine total cells in the sample. Samples were spun down, washed with PBS, and provided to Quantum Labs for graphite furnace analysis to determine total copper in each sample.

Cells were treated as indicated. Afterwards, cells were harvested and lysed. Cell lysates (50 μg) were separated by SDS-PAGE and transferred to a nitrocellulose membrane, followed by visualization using the enhanced chemiluminescence kit. Western blot analysis was performed using specific antibodies to ubiquitin and PARP as described previously. Proteasome inhibition was measured as accumulation of ubiquitinated proteins and apoptosis by cleavage of PARP.

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asked Jun 12, 2019 in Preguntas Comerciales by anonymous

1 Answer

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