Basic Copper Sulphate(WSDTY) Cells were treated with 3 mM DFP and lysed in Triton X-100 lysis buffer containing 20 mM Tris-HCl pH7.4, 1% (v/v) Triton X-100, 150 mM NaCl, 1 mM EDTA, 2 μg/ml leupeptin, 2 μg/ml pepstatin, 10 μg/ml trypsin inhibitor, 44 μg/ml PMSF, 10 μM TLCK and complete mini EDTA-free protease inhibitor. After 10 min incubation on ice, the mixture was centrifuged at 1,000 g for 10 min at 4°C. The supernatant was then used for SDS-PAGE and Western blot detection of active caspase-3, HO-1 or Actin. After incubation under the conditions described above in experimental conditions section, the culture medium was removed, 10 times concentrated with centricon 3 kDa then secretion of pro-MMP1 or ADAMTS5 by chondrocytes was assessed by Western blot.
Wujiang Weishida Copper Technology Co.,Ltd. has 12,000 square meters of production base. By virtue of their sincere cooperation, reciprocity of the business philosophy for the industry users around the country to provide high-quality non-ferrous metal products. Weishida specializes in producing cuprous chloride, basic copper carbonate, copper acetate, copper oxide, copper sulfate. Our company has established a perfect quality assurance system, quality inspection and testing equipment, testing, testing and technology development strength, to ensure the stability of product quality, fully able to meet the pre-market after-sales service to provide users with the need.
Click 8-hydroxyquinoline Copper to learn about more information