Copper Acetate Manufacturers(WSDTY) proposed Levels of copper in the stocks of iron prepared from Sigma Copper Acetate (98% pure) and Alfa Aesar FeCl2 (99.9% pure) were measured at the Caltech Environmental Analysis Center on a Hewlett-Packard 4500 inductively coupled plasma mass spectrometry (ICP-MS) system. Samples of 1 M stock solution were first diluted 1:450 in nitric acid and then sampled. The results were quantified using the standard addition method, by adding pure CuCl2 to each sample to a final concentration of 10, 20, or 30 μg/liter. The lines obtained were extrapolated to the x intercept, which was taken as the amount of copper in the original sample.
The delay in growth caused by the addition of copper or iron was calculated by subtracting the lag phase of the control culture from the lag phase of the metal-treated culture. The synergistic effect of Fe(II) and Cu(II) was calculated by dividing the delay in growth under the Fe(II)-plus-Cu(II) condition by the delay in growth under the Cu(II)-only condition.
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