Beside Basic Copper Sulphate(WSDTY), some other natural compounds less well-known may also display an anti-arthritic activity. For example, ginger root extract Basic Copper Sulphate was shown to decrease pro-inflammatory mediators like Prostaglandin E2 and Nitric oxide (NO) production in arthritic and normal human cartilage slides. Also, copper sulphate promotes collagen synthesis by human articular chondrocytes in vitro. In order to optimize Glucosamine sulphate (GS) efficacy, a new therapeutic association (GSCG) of GS (78.9%), Basic Copper Sulphate (0.105%) and ginger root extract (5.26%) was developed and tested on C-20/A4 chondrocytes and HEK293 T-REx, a commonly used cell line to study basic feature of Nox4 activity. Therefore, the aim of the study was to compare in vitro the potential additional effects of the association of Glucosamine-Copper-Ginger versus Glucosamine alone on the level of ROS production, the MMP expression and chondrocyte apoptosis upon IL-1β stimulation.
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